Zinc deficiency leads to reduced interleukin-2 production by active gene silencing due to enhanced CREM? expression in T cells
نویسندگان
چکیده
Background & aims The micronutrient zinc is essential for proper immune function. Consequently, deficiency leads to impaired function, as seen in decreased secretion of interleukin (IL)-2 by T cells. Although this association has been known since the late 1980s, underlying molecular mechanisms are still unknown. Zinc and reduced IL-2 levels especially found elderly, which turn prone chronic diseases. Here, we describe a new link between expression Methods effects were first investigated vitro human cell lines Jurkat Hut-78 complemented vivo data from zinc-supplemented pigs. A short- long-term model was established. detected flow cytometry profiles on mRNA protein level. Results transcription factor cAMP-responsive-element modulator ? (CREM?) increased during vitro, due phosphatase 2A (PP2A) activity, resulting production. Additionally, supplementation CREM? causing expression. On epigenetic binding promoter mediated histone deacetylase 1 (HDAC1). HDAC1 activity inhibited zinc. Moreover, deacetylation activating mark H3K9 under deficiency, Conclusions With uncovered, connecting with production enhanced PP2A activity.
منابع مشابه
Differential Gene-Expression of Metallothionein 1M and 1G in Response to Zinc in Sertoli TM4 Cells
Background: Zinc (Zn) as an important trace element is essential for testicular development and spermatogenesis. Molecular mechanism of Zn action in the reproductive system may be related to metal binding low-molecular weight proteins, metallothioneins (MT). Our objective was to determine the effect of Zn on two important isoforms of MT, MT1M and MT1G genes expression on testicular sertoli cell...
متن کاملProfile of interleukin-2 production in human mononuclear cells and T-cell lines
Introduction: Interleukin-2 (IL-2) as a T helper type 1 (Th1) cytokine has an important role Background and Objective: Interleukin-2 (IL-2) as a T helper type 1 (Th1) cytokine has an important role in activation, growth and differentiation of several immune cells. Moreover IL-2 is known as a pro-inflammatory and anti-tumoral cytokine. In addition deysregulation of IL-2 in some diseases such as ...
متن کاملSilencing of Survivin Expression Leads to Reduced Proliferation and Cell Cycle Arrest in Cancer Cells
Survivin is an anti-apoptotic gene that is overexpressed in most human tumors. RNA interference using short interfering RNA (siRNA) can be used to specifically inhibit survivin expression. Tumor cells were treated with a newly designed survivin siRNA, which was modified with 2'-OMe. Cellular survivin mRNA and protein levels were determined by real-time qRT-PCR and Western blot, respectively. Ce...
متن کاملInterleukin-7 Optimizes FOXP3+CD4+ Regulatory T Cells Reactivity to Interleukin-2 by Modulating CD25 Expression
The vast majority of Foxp3 regulatory T cells (Treg) exhibits constitutive expression of CD25 (IL-2Rα), which allows the constitution of the high affinity IL-2Rαβγ receptor, ensuring efficient IL-2 binding by Treg. Maintenance of CD25 expression at Treg surface depends on both cell intrinsic factors and environmental stimuli such as IL-2 itself. Whether other factors can participate to maintena...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Nutrition
سال: 2021
ISSN: ['0261-5614', '1532-1983']
DOI: https://doi.org/10.1016/j.clnu.2020.10.052